Assessing Oximetry Response to Chimeric Antigen Receptor T-cell Therapy against Glioma with ¹⁹F MRI in a Murine Model

Purpose To assess the cell-specific, intracellular partial pressure of oxygen (Po2) dynamics both tumor and chimeric antigen receptor (CAR) T cells in a murine immunotherapy model. Materials Methods Human glioblastoma or human were intracellularly labeled with perfluorocarbon nanoemulsion droplet sensors prior to vivo injection severe combined immunodeficient mice measure Po2 two cell types response treatment. Two main sets experiments performed: (a) injected flank perfluorocarbon-labeled then inoculated either CAR untransduced untreated 5 days after inoculation; (b) unlabeled tumors inoculation. Longitudinal fluorine 19 (19F) spin-lattice relaxation time measurements mass used ascertain absolute vivo. Results analyzed for significance using an analysis variance, linear mixed-effect model, Pearson correlation coefficient test, as appropriate. The temporal exhibited delayed, transient hyperoxia at 3 infusion cells, commensurate significant killing T-cell infiltration, observed by bioluminescence imaging histologic findings. Conversely, no changes detected over these same methods. Moreover, it was that total 19F signal quenches treatment, consistent rapid tissue clearance probe from apoptotic cells. Conclusion Cell-specific probes can provide insights into effector function cellular immunotherapeutic cancer models. Keywords: Animal Studies, MR-Imaging, MR-Spectroscopy, Molecular Imaging-Cancer, Imaging-Immunotherapy Supplemental material is available this article. © RSNA, 2021 See also commentary Bulte issue.